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报道了一种将流动注射和毛细管电泳联用的简单、快速、可连续进样的微流体系,该微流体系是用商用毛细管和常规分析实验室易得材料构建,双T构型的微流体系设计是:水平分离通道为内径75μm×146 mm(有效分离长度93 mm)的石英毛细管,毛细管两端由内径0．5 mm的泵管垂直引出,毛细管镶嵌在22mm×6mm×3 mm的有机玻璃基片上,考察了中药样品中的活性组分麻黄碱和伪麻黄碱在双T构型微流体系上的分离行为,比较了流动注射分析仪的两种进样模式,在pH 9．50的15 mmol／L硼砂缓冲溶液、检测波长215 nm、电场强度137 V／cm的条件下,3 min内可获得麻黄碱和伪麻黄碱的基线分离,连续导入一系列样品的进样频率是49 h-1(最大值59 h-1),基于以上实验结果,推导出了计算微流体系进样频率的一种简单的经验公式．
A simple, rapid, and continuously injectable microfluidics system using flow injection coupled with capillary electrophoresis was reported. The microfluidics system was constructed using commercial capillary and readily available materials in a routine analytical laboratory. The flow system is designed as follows: the horizontal separation channel is a quartz capillary with an inner diameter of 75 μm × 146 mm (effective separation length of 93 mm), the capillary tube is led vertically by a pump tube with an inner diameter of 0.5 mm, the capillary tube is embedded in a 22 mm × 6 mm × 3 mm On the plexiglass substrate, the separation behavior of the active components ephedrine and pseudoephedrine in the double-T configuration microfluidic system was investigated. The two injection modes of the flow injection analyzer were compared. At pH 9.50 Baseline separation of ephedrine and pseudoephedrine was achieved within 3 min with a 15 mmol / L borax buffer solution at a detection wavelength of 215 nm and an electric field of 137 V / cm. Sequential introduction of a series of samples with an injection frequency of 49 h-1 (Max. 59 h-1). Based on the above experimental results, a simple empirical formula for calculating the sampling frequency of the microfluidic system is deduced.