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目的研究影响空气净化与消毒装置除菌效果的因素。方法以白色葡萄球菌为指标菌,通过空气消毒试验,分析气溶胶发生器和试验舱温湿度对白色葡萄球菌气溶胶的稳定性影响,并研究微生物气溶胶自然衰减率及初始浓度对评价空气净化与消毒装置除菌效果的影响。结果气溶胶发生器PLG 2000与TK-3发生白色葡萄球菌气溶胶,初始浓度基本都控制在1.0×105cfu/m~3左右,在30 m~3试验舱中1 h的自然衰减均小于30%;试验场温湿度控制在(23±2)℃,(55±5)%时,白色葡萄球菌气溶胶有较好的稳定性;机器性能越好,自然衰减对除菌率的影响越小,反之则影响逐步增大;在一定自然衰减率下,初始浓度越高,除菌率越高,但若初始浓度超过5.0×105cfu/m~3,初始回收的菌落数多不可计。结论气溶胶稳定性是影响空气消毒试验除菌效果评价的关键因素,初始菌落的浓度及回收亦影响除菌率的计算。
Objective To study the factors affecting the germicidal efficacy of air purification and disinfection devices. Methods Staphylococcus aureus was used as an indicator bacteria to determine the effect of temperature and humidity on the stability of Staphylococcus aureus aerosol by air disinfection test. The natural decay rate and initial concentration of microbial aerosols were also evaluated. And disinfection device sterilization effect. Results The initial concentration of Staphylococcus aureus aerosol in aerosol generator PLG 2000 and TK-3 was about 1.0 × 105cfu / m 3. The natural decay of 1 hour in the 30 m 3 experimental cabin was less than 30% ; Staphylococcus aureus aerosol has better stability when temperature and humidity are controlled at (23 ± 2) ℃ and (55 ± 5)%; the better the performance of the machine is, the smaller the effect of natural decay on the sterilization rate is. While the effect is gradually increased; at a certain natural decay rate, the higher the initial concentration, the higher the rate of sterilization, but if the initial concentration exceeds 5.0 × 105cfu / m ~ 3, the number of initial recovery of colonies can not be counted. Conclusion Aerosol stability is the key factor that affects the evaluation of air disinfection test sterilization. The initial colony concentration and recovery also affect the calculation of sterilization rate.