论文部分内容阅读
目的 观察乙型肝炎病毒核心抗原 (HBcAg)核酸疫苗对猕猴的体液和细胞免疫原性。方法 实验组和对照组猕猴分别肌内注射HBcAg核酸疫苗 (pJW 430 3/HBc)和对照质粒 (pJW 430 3)。采用酶联免疫试验检测接种前后猕猴血清中抗 HBc、抗 HBcIgG亚类和猕猴外周血单个核细胞(PBMC)培养上清中干扰素 γ(IFN γ)及白介素 4(IL 4)水平。采用3 H TdR掺入法检测猕猴PBMCHBcAg特异性增殖反应。 结果 实验组猕猴接种该核酸疫苗后均产生了较强的抗 HBc反应。实验组猕猴抗 HBcIgG亚类以IgG2为主 ,PBMC培养上清中IFN γ水平显著增高 ,提示为Th1型免疫应答。实验组猕猴的PBMC抗原特异性增殖反应明显高于对照组。结论 HBcAg核酸疫苗对猕猴具有良好的体液和细胞免疫原性
Objective To observe the humoral and cellular immunogenicity of hepatitis B virus core antigen (HBcAg) nucleic acid vaccine on rhesus monkeys. Methods The experimental group and the control group were injected intramuscularly with HBcAg nucleic acid vaccine (pJW 430 3 / HBc) and control plasmid (pJW 430 3) respectively. The levels of IFN-γ and IL-4 in the culture supernatant of anti-HBc, anti-HBcIgG and cynomolgus peripheral blood mononuclear cells (PBMC) before and after inoculation were detected by enzyme-linked immunosorbent assay. The 3 H TdR incorporation assay was used to detect the PBMC-specific proliferative response in macaque. Results The experimental group of rhesus monkeys vaccinated with the nucleic acid have a strong anti-HBc response. In the experimental group, the anti-HBcIgG subclass of rhesus monkeys was predominantly IgG2, and the IFNγ level in PBMC culture supernatants was significantly increased, suggesting a Th1-type immune response. The PBMC antigen-specific proliferative response in the experimental group was significantly higher than that in the control group. Conclusion HBcAg nucleic acid vaccine has good humoral and cellular immunogenicity to macaques