研究表明 ,外周感染可导致脑源性神经营养因子 (BDNF)的过度表达 ,BDNF可影响其他神经递质的合成。采用免疫组化和原位杂交的方法观察了完全福氏佐剂所致的关节炎大鼠脊髓内 BDNF及其功能性受体酪氨酸蛋白激酶 B(trk B)的表达和促肾上腺皮质激素释放因子 (CRF) m RNA的水平。实验发现 ,在皮下注射完全福氏佐剂后 4h,脊髓腰膨大同侧背角中的 BDNF免疫活性神经元和 CRF m RNA阳性神经元数升高 ,在 2 4 h时达到峰值 ,在 7d时仍维持在较高水平。实验提示 ,关节炎大鼠脊髓内的 BDNF和 CRF可能参与了慢性痛反应。 Studies have shown that peripheral infection can lead to over-expression of brain-derived neurotrophic factor (BDNF), BDNF can affect the synthesis of other neurotransmitters. Immunohistochemistry and in situ hybridization were used to observe the expression of BDNF and its receptor tyrosine kinase B (trk B) in the spinal cord of arthritis rats induced by complete Freund’s adjuvant and the effects of adrenocorticotropic hormone Release factor (CRF) m RNA levels. The results showed that the number of BDNF immunoreactive neurons and CRF m RNA positive neurons in the dorsal horn of lumbar spinal dorsal horn increased 4 h after subcutaneous injection of complete Freund’s adjuvant and peaked at 24 h. Still maintain a high level. Experiments suggest that BDNF and CRF in the spinal cord of arthritis rats may be involved in the chronic pain reaction.