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(3) 混合培养技术: 将植物细胞与藻类或细菌等其它生物细胞混合培养,以促进生长,增加产物。例如:Bradley.P将植物细胞和能固氮的绿色海藻共同培养,Zett.W将诱导植物杀菌素的细菌和植物细胞共同培养等。(4) 生物反应器: 植物细胞悬浮培养的最要重问题之一是如何提高生物反应器的效率。当植物细胞干重超过20g/l时,通气和搅拌便是个大问题。同时,植物细胞比微生动物细胞对剪切应力更敏感。例如:鸡叶柠檬藤在发酵生产期间,其临界剪切应力达到800～2000dyn/cm~2。因此需要开发一种合适的生物反应器,既能保证充足供氧,又能将剪切应力减少到最低限度。
(3) Mixed Culture Technique: Plant cells are mixed with other biological cells such as algae or bacteria to promote growth and increase production. For example: Bradley.P co-cultivates plant cells with nitrogen-fixing green seaweed, and Zett.W co-cultivates bacteriocin-inducing bacteria and plant cells. (4) Bioreactors: One of the most important issues in plant cell suspension culture is how to increase the efficiency of bioreactors. When plant cell dry weight exceeds 20g/l, aeration and stirring is a big problem. At the same time, plant cells are more sensitive to shear stress than micro-animal cells. For example, the critical shear stress of chicken leaf lemon vines reaches 800 to 2000 dyn/cm~2 during fermentation production. Therefore, there is a need to develop a suitable bioreactor that can both ensure adequate oxygen supply and minimize shear stress.