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目的调查15个STR基因座在中国东部蒙古族人群中的基因频率分布。方法应用四色荧光标记引物复合扩增技术,对105名东部蒙古族无关个的血样15个STR基因座进行多态性研究。结果在东部蒙古族人群中15个STR基因座偶合率在0.0084～0.2169之间,个体识别概率(DP)在0.7831～0.9916之间,杂合度在0.5619～0.9231之间,三联非父排除率(PE)在0.4490～0.8444之间,多态性信息总量(PIC)在0.5438～0.9178之间,15个STR基因座总TDP值为0.9999999999998,所有基因座经χ2检验符合Hard-Weinberg平衡。结论上述15个STR基因座在东部蒙古族人群中等位基因分布较好,个体识别率高,适合法医个体识别和亲子鉴定。
Objective To investigate the gene frequency distribution of 15 STR loci in the Mongolian population in eastern China. Methods A total of 15 STR loci in 105 unrelated Mongolian blood samples from eastern China were genotyped by four-color fluorescence-labeled primer multiplex amplification. Results The odds ratios (DPs) of 15 STR loci were between 0.0084 and 0.2169 in the eastern Mongolian population, the odds ratios (DP) ranged from 0.7831 to 0.9916 and the heterozygosities ranged from 0.5619 to 0.9231 ) Ranged from 0.4490 to 0.8444. The total polymorphic information (PIC) ranged from 0.5438 to 0.9178. The total TDP value of 15 STR loci was 0.9999999999998. All loci were in accordance with Hard-Weinberg equilibrium by χ2 test. Conclusion The above 15 STR loci are well distributed in the eastern Mongolian population, with high individual recognition rate, suitable for forensic individual identification and paternity testing.