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应用基因表达谱芯片技术对HCV核心蛋白表达质粒pcDNA3 1(- ) core转染的HepG2细胞和空载体处理的相同细胞差异表达的mRNA进行检测。基因表达谱芯片所检测的 115 2条目的基因均为GenBank中登录的基因 ,HCV核心表达质粒转染的细胞有95条差异表达基因 ,其中 4 5条基因表达增强 ,5 0条基因表达降低。这些核心蛋白反式调节基因与细胞增殖、分化、凋亡、信号转导及免疫调节密切相关。本实验结果为进一步阐明HCV核心蛋白致病 (癌 )的分子生物学机制提供了理论依据。
Genes differentially expressed in HepG2 cells transfected with HCV core protein expression plasmid pcDNA3 1 (-) core and in the same cells treated with empty vector were detected by gene expression profiling. 115 genes detected by gene expression microarray were all registered genes in GenBank. There were 95 differentially expressed genes transfected by HCV core expression plasmids, of which 45 genes were enhanced and 50 genes were decreased. These core protein trans-regulatory genes and cell proliferation, differentiation, apoptosis, signal transduction and immune regulation are closely related. The results of this study provide a theoretical basis for further elucidating the molecular biological mechanism of HCV core protein pathogenesis (cancer).