论文部分内容阅读
用氯化镍对大鼠腹腔注射染毒，以敏感的￣（１２５）Ｉ－后标记新技术检测了血白细胞（ＷＢＣ）和肺组织ＤＮＡ－蛋白质交联（ＤＰＣ）的形成情况。结果显示氯化镍急性染毒后引起ＷＢＣ和肺ＤＰＣ明显升高并有剂量反应关系；小量多次染毒的结果也与１次染毒相一致。表明ＤＰＣ能反映镍化合物对ＷＢＣ和肺器官的遗传毒性，可作为毒作用分子生物标记。结果中还发现白细胞ＤＰＣ的升高较明显并与肺ＤＰＣ有相关性。提示白细胞ＤＰＣ可作为靶器官的替代物反映镍对靶器官的遗传损害。
The rats were injected intraperitoneally with nickel chloride to detect the formation of DNA-protein crosslinks (DPCs) in white blood cells (WBCs) and lungs using a sensitive 125I-labeled technique. The results showed that the acute toxicity of nickel chloride caused a significant increase in WBC and lung DPC and dose-response relationship; the results of a small number of multiple exposure also consistent with a virus exposure. DPC can reflect the genotoxicity of nickel compounds on WBC and lung organs, which can be used as molecular biomarker of toxicity. The results also found that elevated leukocyte DPC more obvious and has a correlation with pulmonary DPC. Tip leukocyte DPC can be used as a target organ substitutes reflect the genetic damage of nickel to target organs.