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目的：探讨ＥＢＶ－ＬＭＰ对人高分化鼻咽癌细胞株ＣＮＥ１增殖能力的影响。方法：以人高分化鼻咽癌细胞株（ＣＮＥ１）为对象，采用电穿孔基因转染技术，将重组ＥＢＶ－ＬＭＰ表达质粒转染ＣＮＥ１细胞。以载体质粒转染及ＣＮＥ１细胞为对照。用细胞体外增殖实验、细胞软琼脂克隆形成率测定、流式细胞仪测定各细胞周期和细胞ＰＣＮＡ的检测，观察细胞增殖能力的变化。结果：体外细胞增殖实验，Ａ比值实验组（２．８６±０．１２）显著高于空白组（１．６２±０．０５）及阴性对照组（１．３８±０．０３）（Ｐ＜０．０１）；实验组细胞软琼脂克隆形成率（２５．２％）显著高于空白组（１１．２％）及阴性对照组（１３．４％）（Ｐ＜０．０１）；ＦＣＭ法测定细胞周期，实验组Ｓ期细胞（３４．９％）高于空白组（２６．２％）及阴性对照组（３０．８％）；ＰＣＮＡ免疫组化染色，实验组细胞ＰＣＮＡ阳性率（８４．６％）明显高于空白组（６１．１％）及阴性对照组（６７．２％）（Ｐ＜０．０１）。结论：ＥＢＶ－ＬＭＰ对ＣＮＥ１细胞体外增殖能力有明显的促进作用，提示ＬＭＰ在ＮＰＣ细胞演进过程中可能起重要作用
Objective: To investigate the effect of EBV-LMP on the proliferation of human highly differentiated nasopharyngeal carcinoma cell line CNE1. Methods: The human EBV-LMP expression vector was transfected into CNE1 cells using human perforin (NPC) cell line (CNE1) as a target by electroporation. The vector plasmid transfected and CNE1 cells as a control. Cell proliferation assay, determination of cell soft agar colony formation rate, determination of cell cycle and PCNA by flow cytometry, and observation of cell proliferation. Results: In vitro cell proliferation assay, the ratio of A to the experimental group (2.86 ± 0.12) was significantly higher than that of the blank group (1.62 ± 0.05) and the negative control group (1.38 ± 0.03) (P < 0.01). The soft agar colony formation rate (25.2%) in experimental group was significantly higher than that in blank group (11.2%) and negative control group (13.4%) (P <0.01) The cell cycle in the experimental group was higher than that in the blank group (26.2%) and the negative control group (30.8%) in the S phase of the experimental group. The positive rate of PCNA in the experimental group was 84% .6%) was significantly higher than the blank group (61.1%) and the negative control group (67.2%) (P <0.01). CONCLUSION: EBV-LMP can significantly promote the proliferation of CNE1 cells in vitro, suggesting that LMP may play an important role in the process of NPC cell proliferation