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目的:探讨胎儿生长受限(fetal growth retriction,FGR)患儿母血及脐血中胎盘生长因子(placenta growth fac-tor,PLGF)浓度与胎盘组织中凋亡相关蛋白Bcl-2、FasL表达的关系。方法:采用双抗体夹心酶联免疫吸附法(ELISA)测定FGR组40例和同期足月正常体重儿(对照组)30例母血、脐血中PLGF浓度;免疫组化SP法检测胎盘组织中Bcl-2,FasL蛋白的表达。结果:①FGR组母血、脐血PLGF浓度与对照组比较,明显降低(P<0.05);②Bcl-2在FGR组胎盘组织的表达比对照组降低,两组相比差异有统计学意义(P<0.05);③FasL在FGR组胎盘组织的表达比对照组降低,两组相比差异有统计学意义(P<0.05);④FGR组母血、脐血中PLGF浓度与胎盘组织中Bcl-2、FasL表达水平呈正相关。结论:母血、脐血PLGF减少可能是引起FGR的原因之一。胎盘组织中Bcl-2表达下降,FasL减弱可能是FGR发病的重要因素之一。在FGR中PLGF可通过上调Bcl-2和FasL的表达,抑制胎盘细胞凋亡,促进胎儿的发育。
Objective: To investigate the relationship between placenta growth fac-tor (PLGF) and the expression of apoptosis-related proteins Bcl-2 and FasL in placenta from fetal growth restriction (FGR) relationship. Methods: The levels of PLGF in maternal serum and umbilical cord blood of 40 FGR patients and 30 full-term normal weight infants (control group) were measured by ELISA. The levels of PLGF in placenta were detected by immunohistochemical SP method Bcl-2, FasL protein expression. Results: (1) PLGF concentration in maternal blood and umbilical cord blood in FGR group was significantly lower than that in control group (P <0.05); ②The expression of Bcl-2 in placenta of FGR group was lower than that in control group <0.05). ③ The expression of FasL in placenta of FGR group was lower than that in control group (P <0.05) .④The PLGF concentration in maternal blood and umbilical cord blood of FGR group was significantly lower than that of Bcl-2, FasL expression was positively correlated. Conclusion: The decrease of PLGF in maternal and umbilical blood may be one of the causes of FGR. Bcl-2 expression in placenta decreased, FasL decreased may be one of the important factors in the pathogenesis of FGR. In FGR, PLGF can inhibit the apoptosis of placental cells and promote the development of fetus by up-regulating the expression of Bcl-2 and FasL.