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目的:通过研究髓系来源的抑制细胞(MDSC)在前列腺癌患者外周血中的分布情况及其特征,探讨MDSC细胞及其亚群与前列腺癌患者预后的相关性。方法:采用流式细胞术检测32例前列腺癌患者及25例健康人外周血中MDSC的比例及其分泌的精氨酸酶1(Arg-1)、iNOS、PD-L1水平,并分析MDSC细胞亚群CD14~+MoMDSC及CD15~+PMN-MDSC的分布情况,以及前列腺癌患者外周血中MDSC及其亚群的水平与患者预后的相关性。结果:与健康人相比,前列腺癌患者外周血中MDSC的比例明显增高(P<0.01),其分泌的Arg-1、iNOS及PD-L1水平亦明显增高(P<0.05),CD14~+Mo-MDSC及CD15~+PMN-MDSC亚群的分布与健康人相比(60.4% vs 72.2%,29.5% vs 18.8%)亦有显著差异(P<0.05),MDSC细胞和Mo-MDSC细胞水平与患者的总生存期具有相关性(P=0.025和0.017)。结论:MDSC细胞及CD14+Mo-MDSC细胞的水平与患者的预后可能存在一定的相关性,这或许为前列腺癌的临床治疗提供了靶点和依据。
OBJECTIVE: To investigate the distribution and characteristics of myeloid derived suppressor cells (MDSCs) in peripheral blood of patients with prostate cancer and to explore the correlation between MDSC cells and their subgroups and the prognosis of patients with prostate cancer. Methods: Flow cytometry was used to detect the percentage of MDSCs and the levels of arginase 1 (Arg-1), iNOS and PD-L1 secreted by peripheral blood from 32 patients with prostate cancer and 25 healthy controls. The distribution of subgroup CD14 ~ + MoMDSC and CD15 ~ + PMN-MDSC, as well as the correlation between the level of MDSC and its subsets and the prognosis of patients with prostate cancer. Results: Compared with healthy controls, the percentage of MDSCs in peripheral blood was significantly increased in patients with prostate cancer (P <0.01), and the levels of Arg-1, iNOS and PD-L1 were also significantly increased (P <0.05) The distributions of Mo-MDSC and CD15 ~ + PMN-MDSC subgroups were also significantly different from those in healthy subjects (60.4% vs 72.2%, 29.5% vs 18.8%, P <0.05) There was a correlation with patient’s overall survival (P = 0.025 and 0.017). Conclusion: There may be some correlation between the level of MDSC cells and CD14 + Mo-MDSC cells and the prognosis of patients, which may provide the target and basis for the clinical treatment of prostate cancer.