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目的 探讨Bax基因在肺癌中的表达及其与细胞凋亡和增殖的关系。方法 对 38例肺癌组织及 2 1例肺部良性组织 ,应用TUNEL技术对细胞凋亡情况进行了检测 ,应用免疫组织化学法对Bax蛋白和增殖细胞核抗原 (PCNA)进行检测。并分别定义凋亡指数和增殖指数。结果 肺癌组织Bax蛋白及PCNA的表达明显高于肺部良性组织 ,P <0 0 1;肺癌组织中凋亡指数和增殖指数均高于肺部良性组织 ,P <0 0 1;而Bax阳性肺癌组织中的凋亡指数明显高于Bax阴性肺癌组织P <0 0 1;结论 Bax蛋白在肺癌中的表达率较高 ,同时在肺癌中细胞凋亡和细胞增殖亦较活跃 ;Bax蛋白过度表达促进细胞凋亡。
Objective To investigate the expression of Bax gene in lung cancer and its relationship with apoptosis and proliferation. Methods Thirty-eight lung cancer tissues and twenty-one benign lung tissues were examined for apoptosis by TUNEL technique. Bax protein and proliferating cell nuclear antigen (PCNA) were detected by immunohistochemistry. Apoptosis index and proliferation index were defined respectively. Results The expressions of Bax protein and PCNA in lung cancer tissues were significantly higher than those in benign lung tissues (P <0.01). The apoptosis index and proliferation index in lung cancer tissues were higher than those in benign lung tissues (P <0.01), while those in Bax positive lung cancer The apoptosis index in the tissue was significantly higher than that in Bax-negative lung cancer (P <0.01) .Conclusion The expression of Bax protein in lung cancer is higher than that in Bax-negative lung cancer, and apoptosis and cell proliferation are also more active in lung cancer. Bax protein overexpression Apoptosis.