论文部分内容阅读
目的建立天王补心丹中棘苷的定性、定量分析方法。方法采用薄层色谱法对天王补心丹中棘苷进行定性鉴别;并用高效液相色谱法测定棘苷的含量。结果在天王补心丹薄层色谱中,展开剂:乙酸乙酯-甲醇-水-冰醋酸(4∶1∶0.5∶0.5),可清晰检出棘苷斑点;HPLC测定天王补心丹中棘苷含量,棘苷线性范围0.0025～0.075g·L-1(r=1.000),平均回收率为100.5%(RSD=1.6%),灵敏度高、结果准确、重现性好。结论方法简便可行,可用于该制剂的质量控制。
Objective To establish a qualitative and quantitative analysis method of Tian Wang Bu Xin Dan Zhong Dang Tang. Methods TLC was used to qualitatively identify the middling glycoside of Tianwangbuxin Danzhong and the content of the saccharin was determined by high performance liquid chromatography. Results In Tianwangbuxin thin-layer chromatography, the eluent: ethyl acetate-methanol-water-glacial acetic acid (4:1:0.5:0.5) could clearly detect the decapitition spots; HPLC determination of Tianwang Buxin Danzhong spine The content of glycosides was linear in the range of 0.0025-0.075 g·L-1 (r=1.000), and the average recovery was 100.5% (RSD=1.6%). The sensitivity was high, the results were accurate, and the reproducibility was good. Conclusion The method is simple and feasible and can be used for the quality control of the preparation.