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目的:通过对人正常输尿管中下段中α_1肾上腺素受体(α_1-AR)亚型mRNA表达情况的研究,了解其各个亚型的分布相对丰度。方法:获取30例病理证实为正常的输尿管中下段,分别取输尿管中段(横跨髂血管处),输尿管下段(输尿管横跨髂血管处与膀胱壁间段之间的中点处),及膀胱壁间段组织,采用实时荧光定量RTPCR方法,检测α_(1a)-AR、α_(1b)-AR及α_(1d)-AR mRNA表达情况。结果:α_1-AR亚型在输尿管中段,下段和壁间段的表达均为α_(1d)-AR>α_(1a)-AR>α_(1b)-AR(42%:32%:26%,51%:26%:23%,56%:24%:20%,P<0.01)。α_(1d)-AR、α_(1α)-AR和_(1b)-AR在输尿管各段的分布均为壁间段>下段>中段(3.8:2.3:1,2.2:1.5:1,2.1:1.6:1,P<0.01)。结论:输尿管中下段存在α_1-AR的各个亚型。从输尿管中段至膀胱壁间段,各受体亚型mRNA表达呈逐渐上升趋势,其中α_(1d)-AR mRNA表达最多。提示以α_(1d)-AR阻滞为主的药物治疗输尿管中下段结石可能比以α_(1a)-AR阻滞为主的药物有更好的疗效。
OBJECTIVE: To investigate the mRNA expression of α_1-adrenergic receptor (α_1-AR) in the lower and middle ureter of normal human being and to find out the relative abundance of its subtypes. Methods: Thirty cases of normal ureter in the middle and lower ureter were obtained, and the middle part of the ureter was taken from the middle part of the ureter (across the iliac vasculature), the lower part of the ureter (the middle of the ureter between the iliac vasculature and the segment of the bladder wall) The expression of α1a-AR, α1b-AR and α1d-AR mRNA were detected by real-time fluorescence quantitative RT-PCR. Results: The expression of α_1-AR subtypes in the middle, lower and middle segments of ureter was α1d -AR> α1a-AR> α1b-AR (42%: 32%: 26% 51%: 26%: 23%, 56%: 24%: 20%, P <0.01). The distributions of α_ (1d) -AR, α_ (1α) -AR and _ (1b) -AR in all segments of the ureter were between the wall segment> the lower segment> the middle segment (3.8: 2.3: 1.6: 1, P <0.01). Conclusion: There are all subtypes of α_1-AR in the middle and lower ureter. From the middle of the ureter to the segment of the bladder wall, mRNA expression of each receptor subtype gradually increased, and the expression of α_ (1d) -AR mRNA was the most. It is suggested that the treatment of middle and lower ureteral calculi with α_ (1d) -AR block may be more effective than α_ (1a) -AR block.