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目的:研究血管紧张素-(1-7)[Ang-(1-7)]对转化生长因子β1(TGF-β1)诱导的大鼠肾小球系膜细胞(GMC)增殖的影响并初步探讨其机制。方法:采用WST法检测培养系膜细胞增殖;RT-PCR法检测细胞内信号传递分子Smad3/7基因mRNA的表达。结果:与对照组比较,Ang-(1-7)明显抑制TGF-β1诱导的GMC增殖(P<0.05);同时,Ang-(1-7)对TGF-β1诱导的系膜细胞内Smad3,Smad7基因mRNA表达有明显的抑制作用(P<0.05)。结论:Ang-(1-7)对TGF-β1诱导的系膜细胞增殖有抑制作用,其机制可能是通过下调细胞内信号传递分子Smad3/7的表达。
AIM: To investigate the effects of angiotensin- (1-7) [Ang- (1-7)] on the proliferation of rat mesangial cells (GMC) induced by transforming growth factor-β1 (TGF-β1) Its mechanism. Methods: The proliferation of mesangial cells was detected by WST method. The expression of Smad3 / 7 mRNA was detected by RT-PCR. Results: Compared with the control group, Ang- (1-7) significantly inhibited the proliferation of GMC induced by TGF-β1 (P <0.05); at the same time, Ang- (1-7) inhibited the proliferation of mesangial cells induced by TGF- Smad7 gene mRNA expression was significantly inhibited (P <0.05). CONCLUSION: Ang- (1-7) can inhibit the proliferation of mesangial cells induced by TGF-β1, and its mechanism may be through down-regulating the expression of intracellular Smad3 / 7.