目的 :建立超临界流体萃取 (SFE)中成药首乌喘息灵中胶囊的大黄素的方法。方法 :正交试验法优选SFE提取工艺 ,并用反相高效液相色谱法进行测定。结果 :在SFE中 ,确定萃取压力为 3 8.5MPa ,温度 60℃ ,改性剂量 0 .4ml,静态萃取时间5min及动态萃取体积 5ml。首乌喘息灵中大黄素的平均回收率为大黄素 97.3 1 %(RSD 1 .2 1 4 %)。结论 :本法是一种快速 ,准确及效率较高的提取方法。 Objective : To establish a method of supercritical fluid extraction (SFE) for the preparation of emodin in Shouwu Xinxiilingzhong Capsule. Methods: The orthogonal test method was used to select the SFE extraction process and the determination was performed by reversed-phase high performance liquid chromatography. RESULTS : In SFE, the extraction pressure was determined as 3 8.5 MPa, the temperature was 60 °C, the modified dose was 0.4 ml, the static extraction time was 5 min and the dynamic extraction volume was 5 ml. The average recovery rate of emodin in Shouwu Xinlingling was 97.31% of emodin (RSD 1.24%). Conclusion: This method is a fast, accurate and efficient extraction method.