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为更好地将保水剂应用到干旱山地的容器苗造林中,以丙烯酰胺-丙烯酸钾交联共聚物型保水剂作为供试材料,设置育苗时(NS)和造林时(AS)各加4.0 g保水剂的两种保水剂应用方式,利用传统的平板计数法和现代的末端限制性片段长度多态性分析(Terminal restricti on fragment length polymorphism,T-RFLP)技术,研究NS和AS对侧柏容器苗造林一年后根际土壤微生物种群结构及对侧柏干旱适应能力的影响.结果表明:保水剂可显著提高侧柏根际土壤细菌、真菌的数量和微生物量碳、氮的含量,且NS处理根际土壤的细菌数量和微生物量碳分别比AS处理提高21.65%和8.04%;保水剂及其应用方式对造林初期侧柏根际土壤的细菌群落结构产生较大影响,保水剂显著增加了根际土壤中细菌群落结构的多样性,NS处理具有最高的丰富度指数、多样性指数和最低的优势度指数;此外,同对照相比,NS处理还显著提高了侧柏容器苗的造林成活率、叶片的净光合速率和相对含水量,降低了相对电导率;同AS处理相比,NS处理的造林成活率和叶片相对含水量分别提高了11.64%和8.33%,而相对电导率降低(降低12.19%).综上说明,保水剂通过改善侧柏容器苗根际土壤的微生物种群结构而提高植物的干旱适应能力,利用添加保水剂的容器苗造林具有更好的应用效果.
In order to better apply the water-retaining agent to container seedling afforestation in arid mountainous areas, acrylamide-potassium acrylate cross-linked copolymer water-retaining agent was used as test material to set the seedling growth (NS) and afforestation (AS) g water retention agent of the application of two kinds of water retention agent, using the traditional plate count method and the modern terminal restriction fragment length polymorphism (Terminal restricti on fragment length polymorphism, T-RFLP) technology to study the NS and AS arborvitae The results showed that the water-retaining agent could significantly increase the amount of bacteria and fungi and the content of microbial biomass carbon and nitrogen in the rhizosphere soil of Arborvitae Miltiorrhizae NS treatment of rhizosphere soil bacteria and microbial biomass carbon were increased by 21.65% and 8.04% than AS treatment; water retention agent and its application on the Arborvitae Rhizosphere soil bacterial community structure had a greater impact, water retention agent significantly increased The diversity of bacterial community structure in rhizosphere soil was the highest. NS treatment had the highest abundance index, diversity index and the lowest dominance index. In addition, compared with the control, NS treatment also significantly raised Compared with AS treatment, the survival rate of afforestation and leaf relative water content of NS treatment increased by 11.64% and 8.33%, while the relative electrical conductivity decreased by 12.19% .In summary, the SAP could improve the plant’s drought adaptability by improving the microbial population structure in the rhizosphere soil of Arborvitae container seedling so that the seedling afforestation with the addition of SAP could have Better application effect.