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用果针入土后４５天左右的花生未成熟子叶为外植体，在含高浓度６－苄基腺嘌呤（ＢＡ）的培养基中，置于约１８００ｌｕｘ光照和２６±１℃条件下，培养２１～２８天，能有效地诱导产生幼芽。其中５号培养基（ＭＳ基本培养基＋ＢＡ２０ｍｇ／１＋ＡＤ１００ｍｇ／ｌ＋蔗糖３％＋琼脂０．８５％），芽诱导率达９２．５％。截取诱导苗带叶腋茎段转接在８号（ＭＳ＋ＢＡ４０ｍｇ／ｌ＋ＹＥ０．０１％）、９号（ＭＳ＋ＢＡ３０ｍｇ／ｌ＋ＹＥ０．０１％＋ＡＤ５０ｍｇ／ｌ）两种培养基中，置于约１８００ｌｕｘ光照和２３～２８℃条件下，培养２１天开始出现丛生芽，４２天后（８）号和（９）号两种培养基均有９５％以上的外植体分化出丛生芽，继而长成无根小苗。平均每个外植体分化出１５个以上小芽，多的达５０个以上。
Immature cotyledons of peanut around 45 days after inoculation with the needles were used as explants and cultured in medium containing 6-benzyladenine (BA) at a high concentration of about 1800 lux and at 26 ± 1 ° C. 21 ~ 28 days, can effectively induce buds. Among them, medium 5 (MS basic medium + BA20mg / 1 + AD100mg / l + sucrose 3% + agar 0.85%) showed a bud induction rate of 92.5%. The transcripts of the axillary stem segments of the seedlings were intercepted in the medium of 8 (MS + BA40mg / l + YE0.01%) and 9 (MS + BA30mg / l + YE0.01% + AD50mg / Under the conditions, clump shoots began to appear on the 21st day after culture, and more than 95% of the explants on the 42th day (8) and (9) both differentiated into clusters of shoots and grew into rootless shoots. An average of more than 15 small buds per explant, more than 50 more.