论文部分内容阅读
ELISA所用的聚苯乙烯板结合蛋白的能力很低。本文报道以硝酸纤维素(NC)为基质的快速斑点免疫结合试验(DIA)检测病毒抗体。作者用10μg/ml补骨脂素衍生物和长波紫外线灭活几种病毒抗原。每种抗原取1μl,滴在8×11cm的NC膜上(1cm间隔)或在印有3mm格子的8.8×8.8cmNC膜上,每3格滴1个抗原,干燥30分钟,将NC膜浸于PBS(含5%脱脂奶粉、0.01%抗泡剂A和0.0001%硫柳汞)中,封闭未结合位点。1小时后,用含0.1%吐温20的PBS洗涤。另将待
The polystyrene plates used for ELISA bind to proteins with low potency. In this paper, nitrocellulose (NC) -based rapid dot immunobinding assay (DIA) was used to detect virus antibodies. The authors inactivated several viral antigens with 10 [mu] g / ml psoralen derivative and UVA. 1 μl of each antigen was dropped on an 8 × 11 cm NC membrane (1 cm interval) or on a 8.8 × 8.8 cmNC membrane coated with 3 mm grids, one antigen per 3 wells, dried for 30 minutes, and the NC membrane was immersed in PBS (containing 5% nonfat dry milk, 0.01% antifoam A and 0.0001% thimerosal), blocking unbound sites. After 1 hour, wash with PBS containing 0.1% Tween20. Will be the other