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按 Chen 和 Howells(1979) 的方法,收集彭亨丝虫感染期幼虫及在哺乳动物体内早期虫体进行体外培养。从埃及伊蚊口部分离获得第3期幼虫移至盛有5毫升0. 05%“Chloro”溶液的生长培养基(GM199) 的消毒离心管内进行虫体表面消毒。GM199为组织培养基加10%新生小牛血清和400微克/毫升晶霉素。幼虫培养在组织培养基199、 Puck培养基、RPMI-1640和 Eagle 基础培养基中,亦使用含有犬肉瘤细胞系、幼仓鼠肾细胞系,仓鼠腹膜细胞系和 HeLA 细胞系。在Falcon25厘米~2的组织培养瓶内含上述各种培养基及细胞系,加50～100条消毒的感染期幼虫作培养实验。
According to the method of Chen and Howells (1979), the larvae of Paenibacillus filaria were collected and the early parasites in mammals were cultured in vitro. The third larvae were isolated from the mouth of Aedes aegypti and transferred to the surface of disinfectant in a sterilized centrifuge tube of growth medium (GM199) containing 5 ml of 0.05% “Chloro” solution. GM199 tissue culture medium plus 10% newborn calf serum and 400 micrograms / ml crystal daimycin. Larvae were cultured in tissue culture medium 199, Puck culture medium, RPMI-1640 and Eagle’s basal medium, also containing the murine sarcoma cell line, baby hamster kidney cell line, hamster peritoneal cell line and HeLA cell line. Falcon25cm ~ 2 tissue culture flask containing the various media and cell lines, plus 50 to 100 disinfection of larvae for culture experiments.