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本研究以优良玉米自交系昌7-2为受体材料,利用农杆菌介导的方法,将带有胚乳特异性表达启动子RP5和淀粉合成关键基因At AGPS的载体p CAMBIA3301-p RP5-At AGPS转入供试玉米材料的茎节中,并对获得的再生植株进行试纸条检测,PCR分子检测以及Southern blotting分析,证明At AGPS基因已经整合到玉米基因组中,转化率约为3.5%。本研究建立了一套优良的玉米遗传转化系统,为玉米分子育种摸索出了一条新的途径。
In this study, Chang 7-2, an elite maize inbred line, was used as the receptor material. Agrobacterium-mediated transformation of p CAMBIA3301-p with the endosperm-specific promoter RP5 and the starch synthesis key gene At AGPS p CAMBIA3301-p RP5- At AGPS was transferred into the stems of the maize material and the test strips, PCR and Southern blotting analysis of the obtained regenerated plants showed that the At AGPS gene has been integrated into the maize genome with a conversion rate of about 3.5% . In this study, we established a set of excellent maize genetic transformation system, which explored a new way for maize molecular breeding.