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目的:建立四倍体盾叶薯蓣人工种子的制作方法,并考察相关影响因素,为四倍体盾叶薯蓣的产业化推广提供技术支持。方法:以四倍体盾叶薯蓣的胚性愈伤作为人工种子的包埋繁殖体,研究人工胚乳组成、种皮基质、贮藏环境、萌发基质等因素对人工种子萌发与成苗的影响。结果:种皮基质为4%海藻酸钠+2%Ca Cl2+2%壳聚糖,胚乳成分为1/2 MS液体培养基+0.2 mg/L BA+0.5 mg/L NAA+0.1 mg/L青霉素+0.3%多菌灵粉剂+0.2%苯甲酸钠+1.0%蔗糖+0.5%活性炭+1.0%木薯淀粉时,人工种子的萌发率和成苗率最高;4℃条件下贮藏20 d后其萌发率及成苗率分别为76.7%、71.7%。结论:以胚性愈伤作为包埋繁殖体的四倍体盾叶薯蓣人工种子制作技术具有生产应用前景。
Objective: To establish a method for making tetraploid Artemisia selengensis dioscorea artificial seeds, and to study the related factors to provide technical support for the industrialization of tetraploid Dioscorea zingiberensis. Methods: Embryogenic callus of tetraploid Dioscorea zingiberensis was used as embedded propagules of artificial seeds to study the effects of artificial endosperm composition, seed coat matrix, storage environment and germination medium on the germination and seedling growth of artificial seeds. Results: The seed coat matrix was 4% sodium alginate + 2% CaCl 2 + 2% chitosan. The endosperm consisted of 1/2 MS liquid medium + 0.2 mg / L BA + 0.5 mg / L NAA + 0.1 mg / L Penicillin + 0.3% carbendazim powder + 0.2% sodium benzoate + 1.0% sucrose + 0.5% activated carbon + 1.0% tapioca starch had the highest germination rate and seedling emergence rate. After 20 d of storage at 4 ℃, the germination rate And seedling rate were 76.7%, 71.7%. Conclusion: Artificial seeds of tetraploid Dioscorea zingiberensis with embryogenic callus as embedded propagules have the potential of production and application.