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以牵牛萌 动种胚为受体，用含有 ３５ Ｓ 启动子— Ｇｕｓ 基因—ｉｐｔ 基因— Ｎｏｓ 基因的根癌农杆菌 Ｌ Ｂ Ａ４４０４ 及含有３５ Ｓ 启动子— Ｇｕｓ 基因—生长素调控基因— Ｎｏｓ 基因的根癌农杆菌 Ｌ Ｂ Ａ４４０４ 进行转化。通过对转化及对照植株苗期叶的同工 酶分析，发现转化植株叶 的过氧化物酶同工酶及酯 酶同 工酶 谱带 与对 照 植株 有明 显差 异。转 化频 率 较高。间 接 验证 了 外源 Ｄ Ｎ Ａ 的导入，说明同工酶分析方法可做为转基因植株早期筛选的方法之一。并初步验证用萌动种胚法转化旋花科植物牵牛的可行性。
Using the germinating embryo of barley as the receptor, Agrobacterium tumefaciens L B A4404 containing 35S promoter - Gus gene - Nos gene and 35S promoter - Gus gene - Ghrelin regulatory gene - Nos gene Agrobacterium tumefaciens L B A4404. Through isozyme analysis of the leaves of the transformed and control plants, the peroxidase isoenzyme and esterase isozyme bands in the leaves of the transformed plants were significantly different from the control plants. High frequency of conversion. Indirect verification of the introduction of exogenous D N A, isozyme analysis methods can be used as one of the early screening methods of transgenic plants. The preliminary validation of the feasibility of transformation of Convolvulaceae plants by germination embryo method.