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目的:研究朊蛋白106-126肽段对分化PC12细胞能量代谢的影响。方法:PC12细胞培养后加入神经生长因子(NGF),再加入朊蛋白106-126肽段,观察细胞存活率、线粒体形态学、线粒体膜电位和Ca2+-ATPase活性的改变。结果:细胞接触肽段后存活率下降28.9%;光镜下可见细胞贴壁不良,胞体缩小,细胞突起断裂缩短;电镜下可见核移位,染色质凝集,部分胞核呈碎块状,线粒体肿胀、嵴紊乱、断裂;膜电位和Ca2+-ATPase的活性随着肽段的作用时间的延长而减小。结论:朊蛋白106-126肽段对分化PC12细胞具有毒性作用,能量代谢障碍可能是其毒性作用机制之一。
Objective: To study the effect of prion 106-126 peptide on the energy metabolism of differentiated PC12 cells. Methods: PC12 cells were cultured with nerve growth factor (NGF), and prion protein 106-126 peptide was added to observe the changes of cell viability, mitochondrial morphology, mitochondrial membrane potential and Ca2 + -ATPase activity. Results: The cell viability decreased 28.9% after exposed to the peptide. The cell adhesion was poor, the cell body was reduced and the protrusion of the cell was shortened. The nuclear translocation and chromatin condensation were observed under electron microscope. Swelling, ridges disorder, fracture; membrane potential and Ca2 + -ATPase activity decreases with the extension of the role of peptides. Conclusion: The prion protein 106-126 peptide has a toxic effect on differentiating PC12 cells. Energy metabolism disorder may be one of the mechanisms of its toxicity.