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In our previous work,a series of polyethylenimine (PEI)-derived cation exchangers were synthesized using PEl-grafted resin FF-PEI-L740 (ionic capacity,740 mmol· L-1) as the basic resin to study lysozyme adsorption and chromatographic behavior.It was found that the resin with an ionic capacity of 630 mmol· L-1 (FF-PEI-CR630) possessed high adsorption performance towards lysozyme at 0-100 mmol· L-1 NaCl.Therefore,in this work,FF-PEI-CR630 was selected to study the influences ofpH and ionic strength (IS) on protein adsorption and chromatographic behavior towards lysozyme.The increase of lysozyme adsorption capacity in the pH range of 6 to 10 was observed.However,the uptake rate decreased in the pH range of 6 to 8 and then remained essentially unchanged from pH 8 to pH 10.Increasing IS led to decreased protein adsorption capacity and increased uptake rate in different pH ranges.Besides,FF-PEI-CR630 maintained dynamic binding capacity as high as over 150 mg· ml-1 at pH 8-10 without NaCl.The research has thus provided insight into the selection of proper pH and IS conditions for protein purification by using FF-PEI-CR630.