【摘 要】
：
甲型肝炎病毒(HAV)在宿主细胞的胞浆内增殖,很少释放到细胞培养的上清液中,因此,给病毒的纯化带来一定困难.本实验室针对制备甲肝诊断试剂盒所需大量病毒抗原问题,对提高抗原
【机 构】
：
中国医学科学院中国协和医科大学医学生物学研究所
论文部分内容阅读
甲型肝炎病毒(HAV)在宿主细胞的胞浆内增殖,很少释放到细胞培养的上清液中,因此,给病毒的纯化带来一定困难.本实验室针对制备甲肝诊断试剂盒所需大量病毒抗原问题,对提高抗原的收获率、浓度和纯度等方法进行改良,建立了以冻融、超声,聚乙二醇(PEG)浓缩,蔗糖/甘油不连续密度梯度离心为主要步骤的快速纯化HAV的方法,并对得到的纯化病毒样品进行相关的性状检测.现将结果报告如下.
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