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１６只成年杂种犬复制心肌缺血再灌注模型，分为地奥心血康（ＤＡＸＸＫ）治疗组和生理盐水（ＮＳ）对照组。结果表明，ＤＡＸＸＫ组血清胞内酶（ＡＳＴ，ＣＫ和ＬＤＨ）水平的增高低于ＮＳ组；心肌细胞膜Ｎａ＋，Ｋ＋－ＡＴＰ酶和Ｍｇ２＋－ＡＴＰ酶比活性高于ＮＳ组；心肌细胞膜ＬＰＯ含量低于ＮＳ组；心肌组织ＳＯＤ水平高于ＮＳ组。心肌电镜观察显示，ＤＡＸＸＫ可维持心肌细胞膜的完整性。＊Ｐ＜０．０１。图２ＤＡＸＸＫ组心肌细胞超微结构（×１２０００倍）细胞膜完整，肌原纤维排列整齐，少数线粒体稍肿胀讨论随着溶栓等疗法的成功应用，发现缺血再灌注可造成组织损伤［６］。寻找有效的抗缺血再灌注损伤的药物，可减轻其对组织损伤的程度。我们的实验证明，ＤＡＸＸＫ可明显降低血清酶水平，在一定程度上保持了膜结构的完整性。心肌超微结构的观察也证明了这一点。心肌缺血再灌注情况下，氧自由基对生物膜的损伤起了很重要的作用［７］。本实验发现，ＤＡＸＸＫ组心肌细胞膜ＬＰＯ含量明显低于ＮＳ组，与维持细胞内环境稳定有关的Ｎａ＋，Ｋ＋－ＡＴＰ酶、Ｍｇ２＋－ＡＴＰ酶比活性，ＤＡＸＸＫ组高于ＮＳ组，并且，Ｎａ＋，Ｋ＋－ＡＴＰ酶比活性与ＬＰＯ含量呈显著的负相关关系。提示由氧自?
Sixteen adult dogs were used to replicate myocardial ischemia-reperfusion model and divided into DAXXK treatment group and NS control group. The results showed that the increase of intracellular enzymes (AST, CK and LDH) in DAXXK group was lower than that in NS group; the specific activity of Na +, K + -ATPase and Mg2 + -ATP in myocardial cell membrane was higher than that in NS group; LPO content in myocardial cell membrane was lower than NS group; myocardial SOD level was higher than NS group. Myocardial electron microscopy showed that DAXXK can maintain the integrity of myocardial cell membrane. * P <0.01. Fig 2 Ultrastructure of cardiomyocytes in DAXXK group (× 12000 folds) Cell membrane integrity, myofibers arranged neatly, and few mitochondria slightly swollen Discussion With the successful application of thrombolysis and other therapies, it was found that ischemia-reperfusion can cause tissue damage [6]. Looking for an effective anti-ischemia reperfusion injury drugs, can reduce the degree of tissue damage. Our experiments show that DAXXK can significantly reduce serum enzyme levels, to some extent, to maintain the integrity of the membrane structure. Myocardial ultrastructure observation also proved this point. In the condition of myocardial ischemia and reperfusion, oxygen free radicals play a very important role in the damage of biofilm [7]. In this experiment, LPO content in DAXXK group was significantly lower than that in NS group. The specific activities of Na +, K + -ATPase and Mg2 + -ATP related to the maintenance of intracellular environmental stability were higher in DAXXK group than those in NS group. Moreover, Na +, K + -ATP enzyme activity and LPO content was significantly negatively correlated. Prompted by the oxygen from?