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病原菌可以利用多种方式激活或者抑制蛋白激酶信号通路来增强其自身侵染力,在病原物与植物互作初期的信号识别和转导中蛋白激酶扮演着重要的角色。已有研究结果表明灰色大角间座壳菌(稻瘟病菌)蛋白激酶基因中含有丰富的SSR。作者通过对灰色大角间座壳菌(稻瘟病菌)基因组中的89个蛋白激酶基因的外显子区、内含子区、5′-UTR和3′-UTR区中的SSR进行了查找和分析,并对编码区中SSR的扩张或收缩对蛋白结构的影响进行了预测。结果表明,SSR在基因的调控区和编码区中的分布是不均一的,且这些基因的外显子区、内含子区、5′-UTR和3′-UTR区中SSR的组成和分布也均不相同;基因的编码区中三碱基和六碱基SSR相对较多,且SSR基序大都表现为GC含量较高,编码的亲水性氨基酸出现的频率也远远高于疏水性氨基酸。通过病菌群体中变异幅度的检测表明,几乎所有的SSR序列都存在多态性。根据灰色大角间座壳菌(稻瘟病菌)自然群体中SSR的变化幅度,对SSR的扩张或收缩对蛋白二级结构的影响进行预测,发现SSR的扩张或收缩都可能对蛋白的结构产生影响。这暗示着SSR的变异在致病相关基因的变异中可能起着十分重要的作用。
Pathogens can use a variety of ways to activate or inhibit the protein kinase signaling pathway to enhance its own infectivity, protein kinases play an important role in signal recognition and transduction of pathogen-plant interactions. Studies have shown that the gene of Pleurotus ostreatus (Magnaporthe grisea) is rich in SSR. The authors looked at the SSRs in the exon, intron, 5’-UTR and 3’-UTR regions of 89 protein kinase genes in the H. armigera (Magnaporthe grisea) genome and The effect of SSR expansion or contraction in the coding region on the protein structure was predicted. The results showed that the distribution of SSR in the regulatory region and coding region of the gene was heterogeneous, and the composition and distribution of SSRs in exon, intron, 5’-UTR and 3’-UTR regions of these genes Are also not the same; gene coding region of the three and six base SSR relatively more, and most of the SSR motifs showed high GC content, encoded hydrophilic amino acids appear much higher frequency of hydrophobic Amino acids. Detection of the magnitude of variation in the germline population showed that almost all SSR sequences are polymorphic. Based on the SSR amplitudes of natural populations of H. armigera (Magnaporthe grisea), the effect of SSR expansion or contraction on the protein secondary structure was predicted and it was found that the expansion or contraction of SSR may have an impact on the structure of the protein . This suggests that the variation of SSR may play a very important role in the variation of disease-related genes.