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目的分析脂多糖(lipopolysaccharide,LPS)对人结肠癌RKO细胞产生的β-防御素3(mouseβ-defensins-3,mBD-3)的影响。方法将不同浓度(0、10、20和40 ng/ml)的LPS作用于RKO细胞0、24、48和72 h,MTT法检测LPS对RKO细胞的最佳抑制浓度;定量RT-PCR及Western blot法检测LPS作用的RKO细胞中mBD-3的表达情况。结果 LPS对RKO细胞的抑制作用在浓度20 ng/ml、培养48 h时最佳。10、20和40 ng/ml LPS作用的RKO细胞,mBD-3 mRNA及蛋白表达水平均明显高于0 ng/ml LPS组,差异有统计学意义(P<0.05)。结论 LPS可以促进mBD-3的表达,为进一步研究mBD-3的产生机制奠定了基础。
Objective To analyze the effect of lipopolysaccharide (LPS) on mouse β-defensins-3 (mBD-3) produced by human colon cancer RKO cells. Methods LPS at different concentrations (0, 10, 20 and 40 ng / ml) were applied to RKO cells for 0, 24, 48 and 72 h. MTT assay was used to determine the optimal inhibitory concentration of LPS on RKO cells. Quantitative RT- blot was used to detect the expression of mBD-3 in RKO cells treated with LPS. Results The inhibitory effect of LPS on RKO cells was best at a concentration of 20 ng / ml for 48 h. The mRNA and protein levels of mBD-3 mRNA and protein in RKO cells treated with 10, 20 and 40 ng / ml LPS were significantly higher than those in 0 ng / ml LPS group, with statistical significance (P <0.05). Conclusion LPS can promote the expression of mBD-3, which lays the foundation for further study on the mechanism of mBD-3 production.