干扰素能增强NK细胞的杀伤活性已见诸多报道.但用人基因工程γ干扰素促进人外周血NK细胞活性的实验,在国内尚未见报道。我们用本所制备的人基因工程γ干扰素(HurIFN-γ)做了这方面的研究。现报告如下。 1 材料与方法 1.1 材料 1.1.1 HurIFN-γ 本室产品,HPLC鉴定纯度>95％。 1.1.2 细胞培养液 RPMI 1640(日水制药株式会社),含10％小牛血消,100U/ml青霉素、100μg/ml链霉素、20mmol/L Hepes以及200mmol/L谷氨酰胺。 Interferon can enhance the killing activity of NK cells has been reported in many reports. But the use of human genetic engineering gamma interferon to promote human peripheral blood NK cell activity experiments have not been reported in the country. We have done this research with human gene-engineered gamma interferon (HurIFN-γ). The report is as follows. 1 Materials and Methods 1.1 Materials 1.1.1 HurIFN-γ product in the laboratory, HPLC purity >95%. 1.1.2 Cell culture solution RPMI 1640 (Nishui Pharmaceutical Co., Ltd.) containing 10% calf blood, 100 U/ml penicillin, 100 μg/ml streptomycin, 20 mmol/L Hepes, and 200 mmol/L glutamine.