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目的研究P63、P504S和Survivin在前列腺病变组织中的表达情况以及三种标记物联合应用的诊断价值。方法采用联合免疫组化的方法,对94例不同前列腺病变组织进行P63、P504S和Survivin联合检测。结果30例前列腺良性增生(BPH)组织基底细胞的细胞核P63染色皆呈阳性,前列腺上皮细胞的胞浆P504S和Survivin染色皆呈阴性。61例前列腺癌(PCa)组织基底细胞的细胞核P63染色均呈阴性,细胞浆P504S染色均呈阳性,其阳性表达率为95.31%(61/64);44例前列腺上皮细胞的胞浆Survivin染色呈阳性(包括两例局部显示阳性),其阳性表达率为68.75%(44/64)。三种标记物在BPH和PCa组织中的阳性表达率存在显著差异(P<0.05)。前列腺癌中P504S阳性表达强度明显高于Survivin,同时发现Survivin在癌术后转移组织中的表达较P504S有增强的趋势。结论P63阴性表达和P504S阳性表达是诊断前列腺癌的重要指标,而Survivin阳性表达趋势可能在鉴别诊断原发性和继发性前列腺癌方面具有重要意义,因此P63、P504S和Survivin联合检测可提高前列腺癌诊断的准确性。
Objective To study the expression of P63, P504S and Survivin in prostatic tissue and the diagnostic value of the combination of three markers. Methods The combined immunohistochemical method of 94 cases of different prostate lesions P63, P504S and Survivin joint detection. Results The nucleus P63 staining of basal cells in 30 benign prostatic hyperplasia (BPH) tissues was positive. The cytoplasm P504S and Survivin staining of prostate epithelial cells were negative. P63 staining was negative in nucleus of basal cells in 61 cases of PCa, positive in cytoplasm P504S staining was 95.31% (61/64), cytoplasm Survivin staining in 44 cases of prostate epithelial cells was Positive (including two cases of local positive), the positive rate was 68.75% (44/64). The positive rates of three markers in BPH and PCa tissues were significantly different (P <0.05). The positive expression of P504S in prostate cancer was significantly higher than that in Survivin, and the expression of Survivin in the metastatic tissues was higher than that in P504S. Conclusion The negative expression of P63 and positive expression of P504S are important indexes in the diagnosis of prostate cancer. However, the positive expression of Survivin may play an important role in the differential diagnosis of primary and secondary prostate cancer. Therefore, the combination of P63, P504S and Survivin can improve the prostate Accuracy of cancer diagnosis.