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以来源于中间偃麦草的八倍体小偃麦为桥梁亲本,通过回交转育的方法,将其所携带的中间偃麦草染色体转移到普通小麦中,从其BC1F6选系中选育出一个高抗小麦秆锈病、白粉病的优良品系CH08-141,并利用分子细胞学方法对其进行鉴定。以中间偃麦草基因组DNA为探针的GISH结果表明,CH08-141中包含1对来源于中间偃麦草的染色体,属于小麦-中间偃麦草异代换系;以平均分布于小麦基因组染色体上的48对SSR引物对CH08-141分子标记染色体定位,结果表明,CH08-141中的6B染色体被中间偃麦草的1对J组染色体所代换。新鉴定的异代换系含有来源于中间偃麦草的抗秆锈病和白粉病基因,是小麦抗病育种中不可多得的新抗源。
Triticum aestivum triticale from the parents of the parents of the bridge, by backcrossing methods, will carry the Chromosomes of the Thinopyrum intermedium to transfer to wheat, from its BC1F6 line selected a High resistance to wheat stem rust, powdery mildew of the excellent line CH08-141, and the use of molecular cytology methods to identify them. GISH results using T. triticina genome DNA as probes revealed that CH08-141 contains one pair of chromosomes derived from Thinopyrum intermedium and belongs to the wheat-Thinopyrum intermedium substitutional substitution system; Chromosome mapping of CH08-141 by SSR primer showed that the chromosome 6B of CH08-141 was replaced by one pair of J chromosome of T. triticina. The newly identified allelic substitution lines contain the genes for resistance to stem rust and powdery mildew from Thinopyrum intermedium, which are rare new resistance sources in wheat disease resistance breeding.