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目的采用代谢组学技术寻找鼻咽癌诊断和治疗的标志物。方法采用气-质联用(GS-MS)技术,检测了49例鼻咽癌患者(Ⅰ期19例,Ⅱ期17例,Ⅲ期13例),37例喉癌患者和40个健康人血清中52种内源性代谢物。结果代谢谱比较分析结果显示,犬氨酸、N-乙酰葡萄酰胺、硬脂酸、甘氨酸、N-乙酰半乳糖胺、N-乙酰葡萄胺和对羟基丙酮酸在鼻咽癌患者血清中显著升高(～3倍)(P<0.05),其中犬氨酸、N-乙酰葡萄酰胺、N-乙酰半乳糖胺、N-乙酰葡萄胺和对羟基丙酮酸在鼻咽癌Ⅰ到Ⅲ逐步升高(P<0.05),治疗后该5种内源性代谢物逐步降低,且恢复到正常水平(P<0.05)。其中4种代谢物犬氨酸、N-乙酰葡萄酰胺、N-乙酰葡萄胺和对羟基丙酮酸水平降低与鼻咽癌治疗后肿瘤减小率成正相关,因此其可作为鼻咽癌潜在标志物。此外,这4个代谢物还与鼻咽癌复发转移有关。结论犬氨酸、N-乙酰葡萄酰胺、N-乙酰葡萄胺和对羟基丙酮酸是鼻咽癌变和侵袭重要的代谢产物。
Objective To use metabonomics techniques to find the markers of nasopharyngeal carcinoma diagnosis and treatment. Methods 49 patients with nasopharyngeal carcinoma (19 cases in stage Ⅰ, 17 cases in stage Ⅱ, 13 cases in stage Ⅲ), 37 cases of laryngeal cancer and 40 healthy persons were detected by gas chromatography-mass spectrometry (GC-MS) In 52 kinds of endogenous metabolites. Results The results of metabolic profiling showed that the contents of kinine, N-acetylglucosamine, stearic acid, glycine, N-acetylgalactosamine, N-acetylglucosamine and p-hydroxypyruvate were significantly increased in patients with nasopharyngeal carcinoma (~ 3 times) (P <0.05). The levels of kynurenine, N-acetylglucosamine, N-acetylgalactosamine, N-acetylglucosamine and p- (P <0.05). After treatment, the five endogenous metabolites gradually decreased and returned to normal levels (P <0.05). The four metabolites, kin, N-acetylglucosamine, N-acetylglucosamine and p-hydroxy-pyruvate levels decreased with the tumor reduction rate of nasopharyngeal carcinoma is positively correlated, it can be used as a potential biomarker for nasopharyngeal carcinoma . In addition, these four metabolites are also associated with recurrence and metastasis of nasopharyngeal carcinoma. Conclusion The results showed that kynurenine, N-acetylglucosamine, N-acetylglucosamine and p-hydroxy-pyruvate are important metabolites of nasopharyngeal carcinogenesis and invasion.