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目的研究面粉增白剂过氧化苯甲酰(benzoyl peroxide,BPO)对小鼠肾脏的毒性。方法将64只清洁级昆明小鼠随机分成4组,分别为对照(玉米油)组和低(50 mg/kg)、中(100 mg/kg)、高(200 mg/kg)剂量BPO染毒组,每组16只,雌雄各半。采用经口灌胃的方式进行染毒,染毒容量为5 ml/kg,每天1次,连续染毒6周。染毒结束后,测定肾组织匀浆上清液中的超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,定量观察坏死细胞与梗死灶数目,并计算坏死细胞率。结果与对照组相比,仅高剂量BPO染毒组小鼠SOD活力降低,MDA含量增高,差异均有统计学意义(P<0.05)。低、中、高剂量BPO染毒组小鼠肾脏细胞坏死率均高于对照组,差异均有统计学意义(P<0.05)。高剂量BPO染毒组坏死细胞率高于低、中剂量BPO染毒组,差异均有统计学意义(P<0.05)。高剂量组16只小鼠病理切片共观察到13处梗死灶。结论在一定剂量范围内,BPO可以损伤小鼠体内的抗氧化系统,导致肾脏出现不同程度的氧化损伤。
Objective To study the toxicity of flour whitening agent benzoyl peroxide (BPO) to the kidneys of mice. Methods Totally 64 clean Kunming mice were randomly divided into 4 groups: control (corn oil) group and low (50 mg / kg) and medium (100 mg / kg) and high (200 mg / kg) Group, 16 in each group, male and female. The rats were orally administered by oral gavage at a volume of 5 ml / kg once daily for 6 weeks. After the exposure, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the supernatant of renal tissue were measured. The number of necrotic cells and infarct were quantitatively observed, and the necrotic cell rate was calculated. Results Compared with the control group, the SOD activity and the content of MDA in high dose BPO group were significantly decreased (P <0.05). The renal necrosis rate of mice in low, medium and high doses of BPO was higher than that of the control group, the differences were statistically significant (P <0.05). The necrosis rate of high dose BPO group was higher than that of low and medium dose BPO group (P <0.05). High-dose group of 16 mice pathological sections were observed in 13 infarction. Conclusion BPO can damage the antioxidant system in mice at certain dose range, resulting in varying degrees of oxidative damage in the kidney.