[Objective] The pollen viability of honeysuckle was determined at different storage conditions, which provided a reference for the production and research of honeysuckle. [Method] Taking the fresh pollen of honeysuckle as an experimental material, Ⅰ-KⅠ staining, TTC staining and culture medium germination methods were used to determine its fresh pollen ability. Effects of different storage conditions on the pollen germination were studied. [Result] The results showed that adding different concentrations of sucrose and boric acid to culture media had promoted effects on the pollen germination. When the concentrations were 150 and 30 mg/L, the germination rate reached the maximum value of 75.23% and 52.78%. Ⅰ-KⅠ staining was not suitable for the determination of pollen viability of honeysuckle, while TTC staining was suitable for it and the rate reached 80.46%. [Conclusion] This method is relatively accurate and simple. It is better for mid-low temperature storage(-80 ℃) than cold storage(4 ℃, -23 ℃), and it is not suitable for the normal condition. [Objective] The pollen viability of honeysuckle was determined at different storage conditions, which provided a reference for the production and research of honeysuckle. [Method] Taking the fresh pollen of honeysuckle as an experimental material, I-KI staining, TTC staining and culture effect of different storage conditions on the pollen germination were studied. [Result] The results showed that adding different concentrations of sucrose and boric acid to culture media had promoted effects on the pollen germination. When the concentrations were 150 and 30 mg / L, the germination rate reached the maximum value of 75.23% and 52.78%. I-KI staining was not suitable for the determination of pollen viability of honeysuckle, while TTC staining was suitable for it and the rate reached 80.46%. [Conclusion] This method is relatively accurate and simple. It is better for mid-low temperature storage (-80 ° C) than c old storage (4 ° C, -23 ° C), and it is not suitable for the normal condition.