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目的 研究大肠癌患者外周血树突状细胞（ Ｄ Ｃ）体外诱导抗肿瘤免疫反应能否预防裸鼠移植瘤发生及抑制裸鼠移植瘤生长。方法 联合应用粒／巨噬细胞集落刺激因子（ Ｇ Ｍ Ｃ Ｓ Ｆ）及白介素４（ Ｉ Ｌ４）从大肠癌患者外周血中培养出 Ｄ Ｃ；人大肠癌细胞系 Ｌ Ｏ Ｖ Ｏ 细胞的肿瘤抗原粗提物刺激 Ｄ Ｃ； Ｄ Ｃ激活同源的 Ｔ 淋巴细胞； Ｄ Ｃ激活的 Ｔ 淋巴细胞预防性接种于裸鼠皮下，观察随后接种的人大肠癌细胞系 Ｌ Ｏ Ｖ Ｏ 移植瘤发生率；观察 Ｄ Ｃ 激活的 Ｔ 淋巴细胞抑制已接种于裸鼠的人大肠癌细胞系 Ｌ Ｏ Ｖ Ｏ 移植瘤生长。结果 Ｄ Ｃ 体外激活的 Ｔ 淋巴细胞明显能预防裸鼠人大肠癌细胞系 Ｌ Ｏ Ｖ Ｏ 移植瘤发生（预防组 １０％ ， 对照组 １００％ ， Ｐ＜ ０００１）； Ｄ Ｃ 体外激活的 Ｔ 淋巴细胞抑制该移植瘤生长〔对照组、治疗组 及加强 治疗组 肿瘤 大小分 别为 ８７３ｍ ｍ ２ ±１９ｍ ｍ ２ 、５６９ｍ ｍ ２ ±１７ｍ ｍ ２ 、３６１ｍ ｍ ２ ±２６ｍ ｍ ２ ， Ｐ＜ ００５ 及 Ｐ＜ ００１〕。结论 大肠癌患者外周血 Ｄ Ｃ体外诱导的抗肿瘤细胞免疫反应不仅能预防人大肠癌细胞系 Ｌ Ｏ Ｖ Ｏ 裸鼠移植瘤发生，而且能抑制该移植瘤生长
Objective To investigate whether the induction of anti-tumor immune responses by dendritic cells (DCs) in peripheral blood of patients with colorectal cancer can prevent the occurrence of transplanted tumors in nude mice and inhibit the growth of transplanted tumors in nude mice. Methods D C was cultured from the peripheral blood of patients with colorectal cancer in combination with granulocyte/macrophage colony-stimulating factor (GMSSF) and interleukin-4 (IL-4); human colorectal cancer cell line L O V O The crude extract of tumor antigens from cells stimulates D C; D C activates homologous T lymphocytes; D C-activated T lymphocytes are inoculated subcutaneously in nude mice and the subsequently inoculated human colorectal cancer cell line L O V O is observed. The incidence of xenografts; D C-activated T lymphocytes were observed to inhibit the growth of transplanted tumors of human colorectal cancer cell line L O V O that had been inoculated in nude mice. Results D C in vitro activated T lymphocytes could significantly prevent the occurrence of L O V O tumor in human colorectal cancer cell lines in nude mice (10% in the prevention group, 100% in the control group, P < 0001); D C activation in vitro T-lymphocytes inhibited the growth of the transplanted tumors. The tumor sizes in the control, treatment, and booster groups were 873m m 2 ±19m m 2, 569m m 2 ±17m m 2, and 361m m 2 ±26m m 2, respectively. P<0. 05 and P< 001〕. Conclusion The anti-tumor immune response induced by D C in peripheral blood of patients with colorectal cancer can not only prevent the occurrence of transplanted tumors of human colorectal cancer cell line L O V O in nude mice, but also inhibit the growth of this tumor.