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目的利用微悬臂梁传感技术对瘦肉精抗原抗体的特异性结合进行检测。方法将巯基化的瘦肉精抗体通过自组装修饰到微悬臂梁的金面,在加入不同浓度的瘦肉精标准样品的过程中,通过光杠杆原理监测微悬臂梁的实时弯曲信号。巯基化瘦肉精抗体的活性和微悬臂梁上抗原抗体的结合得到酶联免疫吸附(ELISA)实验的验证。结果该系统能够检测至少1μg/L浓度的瘦肉精标准样品,而且具有很强的选择性,在对照实验中加入不含瘦肉精的1 mg/L氯霉素溶液没有响应。另外,瘦肉精抗原抗体的结合导致微悬臂梁产生压应力,而且微悬臂梁表面应力的改变与样品浓度的对数成线性关系。结论利用微悬臂梁传感技术对瘦肉精的检测是可行的。
Objective To detect the specific binding of leptin antigen antibody using micro-cantilever sensing technique. Methods The thiolated clenbuterol antibody was modified to the gold surface of micro-cantilever by self-assembly method. The real-time bending signal of micro-cantilever was monitored by light lever principle during the adding of different concentrations of lean-sperm standard sample. Enzyme linked immunosorbent assay (ELISA) was used to verify the binding of thiolated clenbuterol antibody to the antigen on the micro-cantilever. Results The system was able to detect standard lean meat extract with a concentration of at least 1 μg / L and was highly selective. In the control experiment, 1 mg / L chloramphenicol solution without leanin was unresponsive. In addition, binding of leptin antigen antibody results in compressive stress on the microcantilever, and the change in surface stress on the microcantilever is linearly related to the logarithm of sample concentration. Conclusions The detection of lean meat extract using micro-cantilever sensing technique is feasible.