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目的:本文将对腹泻患儿进行肠致病性大肠埃希菌病原学检验,从而探讨肠致病性大肠埃希菌的病原学特点。方法:对所有腹泻患者进行粪便采集样本,之后应用病原学检验方法对样本中肠致病性大肠埃希菌进行分离,给予病原学检查,包括形态特征分析、培养特征分析以及生化反应特征分析等,得出结论。结果:231例腹泻患儿粪便样本中均检测出肠道致病性大肠埃希菌,检出率为100.00%。肠道致病性大肠埃希菌菌落呈现出灰色,可略带白色,菌落具有整齐的边缘以及光泽表面;对肠致病性大肠埃希菌进行革兰氏染色可知,其结果为阴性,无芽孢出现,但出现鞭毛,属于短小杆菌,两端呈现出圆顿形。结论:研究肠致病性大肠埃希菌的病原学特点能够有效提高肠道致病性大肠埃希菌引发腹泻等疾病的确诊率,使患者及时得到具有针对性的治疗措施,提高患者治疗效果。
Objective: In this paper, diarrhea in children with enteric pathogenic Escherichia coli pathogenic test to explore the pathogenic characteristics of intestinal pathogenic Escherichia coli. Methods: All patients with diarrhea were collected samples of feces, and then pathogenicity test was used to separate the enteropathogenic Escherichia coli in the samples. Pathological examination was conducted, including morphological characteristics, culture characteristics and biochemical reaction characteristics analysis ,get conclusion. Results: The intestinal pathogenic Escherichia coli was detected in all the 231 cases of diarrhea in children. The detection rate was 100.00%. Intestinal pathogenic Escherichia coli colonies appear gray, slightly white, colonies with a neat edge and a glossy surface; Gram stain of enteropathogenic Escherichia coli, the result is negative, no Spores appear, but flagella appear, are short bacillus, both ends showed a round shape. Conclusion: The etiological characteristics of enteropathogenic Escherichia coli can effectively improve the diagnosis of intestinal pathogenic Escherichia coli-induced diarrhea and other diseases, so that patients receive targeted treatment in time and improve the treatment effect of patients .