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目的 :探讨绞股蓝总皂甙 (GPS)对注射内毒素的家兔血流动力学和 NO的影响。方法 :动物随机分 3组 :对照组 ,内毒素 (L PS)组及 GPS+L PS组。动物静脉麻醉后 ,取血测量 NO水平 ;通过二道生理记录仪和 SMUP- A型生物信号处理仪测量血流动力学指标。动物处死后 ,取血再次测量 NO水平。结果 :对照组实验前后各指标没有明显的变化 (P>0 .0 5 )。 L PS组平均动脉血压 (MAP)、心率、心室内压峰值、± dp/ dtmax和心力环面积在实验后 2 h时没有出现明显的变化 ,但在实验后 4h、6 h时明显低于实验前和对照组 (P<0 .0 1) ,实验后 NO- 2 明显高于实验前。GPS组实验后 2 h,4h时 MAP、心率、心室内压峰值、± dp / dtmax和心力环面积与实验前及对照组比较 ,没有明显差异 (P>0 .0 5 ) ,但在 4h时明显高于 L PS组 (P<0 .0 1) ;实验后 6 h时 ,MAP、心率、心室内压峰值、± dp/ dtmax和心力环面积低于实验前和对照组 (P<0 .0 5 ) ,但高于 L PS组 (P<0 .0 5 ) ;实验后的 NO- 2 浓度高于实验前和对照组 (P<0 .0 5 ) ,但低于 L PS组实验后的 NO- 2 浓度 (P<0 .0 5 )。结论 :绞股蓝总皂甙对注射内毒素的家兔血流动力学有保护作用和抑制一氧化氮大量释放的作用
Objective: To investigate the effect of gypenosides (GPS) on hemodynamics and NO in rabbits injected with endotoxin. Methods: Animals were randomly divided into 3 groups: control group, endotoxin (L PS) group and GPS+L PS group. Animals were anesthetized and blood was taken to measure NO levels; hemodynamic parameters were measured by a two-channel physiological recorder and a SMUP-A biological signal processor. After the animals were sacrificed, blood was taken again to measure NO levels. Results: There was no significant change in the index before and after the control group (P>0.05). The mean arterial blood pressure (MAP), heart rate, peak ventricle pressure, ±dp/dtmax, and cardiac ring area did not change significantly in the L PS group at 2 h after the experiment, but were significantly lower than the experiment at 4 h and 6 h after the experiment. Before and in the control group (P<0.01), the NO-2 was significantly higher after the experiment than before the experiment. There was no significant difference in MAP, heart rate, peak ventricle pressure, ± dp / dtmax, and cardiac ring area between the two groups at 2 h and 4 h after the GPS group (P>0.05), but at 4h Significantly higher than L PS group (P <0. 1); 6 h after the experiment, MAP, heart rate, peak intraventricular pressure, ± dp/dtmax, and cardiac ring area were lower than before the experiment and the control group (P <0. 0 5 ), but higher than L PS group (P 0.05); NO 2 concentration after the experiment was higher than before the experiment and the control group (P 0.05), but lower than the L PS group after the experiment NO-2 concentration (P<0.05). Conclusion : Gynostemma total saponin can protect hemodynamics and inhibit the release of nitric oxide in rabbits injected with endotoxin