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先在硅胶表面接枝偶氮活性基团,在此基础以去氧肾上腺素(Phenylephrine,PE)为替代模板分子、丙烯酰胺(Acrylamide,AM)为功能单体在其表面合成具有特定识别克伦特罗(Clenbuterol,CLB)分子印迹聚合物。计算机分子模拟确定了功能单体与模板分子最佳比例为3:1;对各步合成的硅胶进行了表征证明成功合成了聚合物;通过对比不同反应条件下所得聚合物对CLB吸附率筛选出了最佳聚合反应条件(65℃,12 h);对不同浓度梯度CLB标准液进行高效液相色谱分析,绘制出标准曲线,并以此分别测定CLB标准液低速流入所合成聚合物为填料的固相萃取柱的浓度差计算聚合物的吸附率;最后通过对比不加模板所合成聚合物和硅胶对CLB的吸附率,证明了所合成聚合物具有特异识别CLB能力。合成的聚合物可用于检测克伦特罗时的样品前处理,或作为传感元件进一步研发快速检测克仑特罗的传感器。
Firstly, an azo active group was grafted on the surface of silica gel, on the basis of Phenylephrine (PE) as a template molecule and Acrylamide (AM) as a functional monomer to synthesize a monomer with specific recognition of Karen Clobuterol (CLB) molecularly imprinted polymers. Computer molecular modeling confirmed that the optimal ratio of functional monomer to template molecule was 3: 1. The silica synthesized by each step proved that the polymer was successfully synthesized. By comparing the adsorption rate of CLB obtained under different reaction conditions (65 ℃, 12 h). The standard curve of CLB standard solution with different concentration gradient was drawn and the standard curve was drawn. Then, the CLB standard solution was measured for low-speed influx of the synthesized polymer as filler The concentration of solid phase extraction column was used to calculate the adsorption rate of polymer. Finally, by comparing the adsorption rate of CLB with the polymer and silica gel without template, it was proved that the synthesized polymer has the ability of specifically recognizing CLB. Synthetic polymers can be used for sample preparation when clenbuterol is detected, or as a sensing element for further development of Clenbuterol-based sensors.