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目的:观察脑缺血再灌注时小胶质细胞的活化和外源性巨噬细胞来源。方法:阻塞大鼠大脑中动脉2h再灌注0.5～48h制成脑缺血模型,凝集素GSI-B4组化法观察小胶质细胞/巨噬细胞在脑组织的分布、免疫组化观察室管膜细胞增殖及分化。结果:再灌注0.5h,可见大量形态各异的GSI-B4阳性的小胶质细胞与巨噬细胞,随后下降,12～48h数量持续增高,并呈不同的形态。各实验组,室管膜及室管膜下层有细胞增殖核抗原(proliferationcellnuclearantigen,PCNA)强阳性细胞。杏仁核、杏仁皮质核、视前区及其软脑膜有PCNA强阳性细胞和GSI-B4强阳性阿米巴样巨噬细胞。再灌注24h组,有PCNA与白细胞共同抗原45RB(leukocytecommonantigen,CD45RB)双标阳性的细胞。结论:缺血再灌注时,不同损伤区域小胶质细胞形态呈明显的异形性,外源性巨噬细胞可能来自脑膜巨噬细胞和室管膜及室管膜下层的增生、浸润和迁移。
Objective: To observe the activation of microglia and the origin of exogenous macrophages during cerebral ischemia-reperfusion. Methods: Cerebral ischemia models were occluded 2 h after reperfusion of middle cerebral artery occlusion rats and 0.5-48 h reperfusion. The distribution of microglial cells / macrophages in brain tissue was observed by agglutinin GSI-B4 staining. Membrane cell proliferation and differentiation. Results: After reperfusion for 0.5h, a large number of GSI-B4 positive microglial cells and macrophages were observed, and then decreased. The number of GSI-B4-positive microglia and macrophages continued to increase after 12-48 hours of reperfusion, and showed different morphologies. Each experimental group, ependymal and subependymal cells have cell proliferating nuclear antigen (proliferating cell nuclear antigen (PCNA) strong positive cells. There were PCNA positive cells and GSI-B4 strongly positive amoebic macrophages in the amygdala, almond cortex nucleus, preoptic area and the pia mater. Reperfusion 24h group, PCNA and white blood cell common antigen 45RB (leukocytecommonantigen, CD45RB) double positive cells. CONCLUSION: During ischemia / reperfusion, microglial morphology in different injury regions shows obvious heteromorphism. Exogenous macrophages may be derived from the proliferation, infiltration and migration of the macrophages and ependymal and subventricular layers.