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目的检测急性白血病患儿白血病干细胞(LSC)的表面分子CD96的表达,并分析其临床意义。方法分离骨髓中单个核细胞,在此基础上采用分选型流式细胞仪分离CD34+CD38-CD123+的LSC进行培养,采用检测型流式细胞仪检测69例初治急性白血病患儿LSC表面分子CD96的表达,利用R显带技术检测69例患儿染色体核型,收集患儿血常规,检测免疫相关指标等资料。结果 CD96主要在急性髓系白血病(AML)中表达,在急性淋巴细胞白血病(ALL)中也有少量表达,二者之间的表达差异有统计学意义(P<0.05)。维吾尔族患儿CD96的中位表达含量23.4%,汉族患儿CD96的中位表达含量21.2%,表达量在两民族之间差异无统计学意义(P>0.05)。CD96+患儿多伴有预后不良的染色体核型。CD96+患儿初次化疗完全缓解率低于CD96-患儿,而化疗后感染率及复发率明显高于CD96-患儿,差异有统计学意义(P<0.05)。结论急性白血病LSC表面CD96表达阳性的患儿预后欠佳,CD96分子可能是判断急性白血病预后的一个新型指标。
Objective To detect the expression of surface molecule CD96 on leukemia stem cells (LSC) in children with acute leukemia and analyze its clinical significance. METHODS: Mononuclear cells from bone marrow were isolated and sorted by LSC on CD34 + CD38-CD123 + by flow cytometry. LSC surface molecules of 69 newly diagnosed acute leukemia children were detected by flow cytometry CD96 expression, the use of R-banding detection of chromosomal karyotype in 69 children with blood routine collection, detection of immune-related indicators and other data. Results CD96 was mainly expressed in acute myeloid leukemia (AML) and a small amount in acute lymphoblastic leukemia (ALL), the difference was statistically significant (P <0.05). The median expression level of CD96 was 23.4% in Uighur children and 21.2% in Han nationality. There was no significant difference in expression level of CD96 between the two ethnic groups (P> 0.05). CD96 + patients with more often associated with poor prognosis of the karyotype. The complete remission rate of primary chemotherapy in CD96 + children was lower than that of CD96- children, and the infection rate and recurrence rate after chemotherapy were significantly higher than those of CD96- children. The difference was statistically significant (P <0.05). Conclusions The prognosis of acute leukemia in children with positive expression of CD96 on LSC is poor. The expression of CD96 may be a new index to judge the prognosis of acute leukemia.