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目的:探讨肿瘤坏死因子α(TNF-α)和肿瘤坏死因子β(TNF-β)基因多态性与多发性骨髓瘤(MM)易感性的关系。方法:采用聚合酶链反应-连接测序技术检测86例MM患者和172例健康对照者TNF-α(TNF-α-857、TNF-α-308和TNF-α-238位点)和TNF-β(TNF-β+252位点)基因多态性,以非条件Logistic回归分析计算比值比(OR)及95%可信区间(CI),从而评估不同基因型与MM风险之间的相关性。结果:MM组TNF-α-857TT基因型及T等位基因频率显著高于对照组,病例组与对照组TT基因型频率分别为5.8%和1.2%,T等位基因频率分别为17.4%和9.6%。携带TNF-α-857TT型者发生MM风险增高为5.78倍(OR=5.78,95%CI:1.09～30.6,P=0.039),携带TNF-α-857T等位基因者发生MM风险增高为1.99倍(OR=1.99,95%CI:1.17～3.39,P=0.011),其他基因位点多态性未见与MM具有显著相关性。结论:在目前样本条件下,TNF-α-857位点T等位基因或TT基因型与MM发病风险增高具有相关性。
Objective: To investigate the relationship between the polymorphisms of tumor necrosis factor α (TNF-α) and tumor necrosis factor β (TNF-β) and the susceptibility of multiple myeloma (MM). Methods: The expression of TNF-α (TNF-α-857, TNF-α-308 and TNF-α-238) and TNF-β in 86 MM patients and 172 healthy controls were detected by polymerase chain reaction- (TNF-β + 252) gene polymorphisms. The odds ratio (OR) and 95% confidence interval (CI) were calculated by non-conditional Logistic regression to evaluate the association between different genotypes and MM risk. Results: The frequencies of TNF-α-857TT genotype and T allele in MM group were significantly higher than those in control group. The frequencies of TT genotypes in cases and controls were 5.8% and 1.2%, respectively. The frequencies of T allele were 17.4% and 9.6%. The risk of developing MM with TNF-α-857TT increased by 5.78-fold (OR = 5.78, 95% CI: 1.09-30.6, P = 0.039) (OR = 1.99, 95% CI: 1.17-3.39, P = 0.011). There was no significant correlation between other polymorphisms and MM. Conclusion: Under the current sample conditions, there is a correlation between T allele of TNF-α-857 locus and TT genotype and increased risk of MM.