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目的:小陷胸加枳实汤有效成分提取工艺及其抗菌作用。方法:采用正交试验,HPLC分析技术及抑菌试验等对小陷胸加枳实汤有效成分的提取工艺、抑菌作用等进行研究。结果:与水配量比1∶4、提取时间1.5h、提取次数3次为较佳提取工艺条件;抑菌MIC:霉菌>125 g·L-1,大肠埃希菌、金黄色葡萄球菌>62.5 g·L-1。结论:采用合煎水提取工艺提取小陷胸加枳实汤有效成分工艺的较佳条件为A1B3C3,提取物均具有一定的抗菌作用。
Objective: The extraction technology and antibacterial effect of active ingredients of small chest-debriding and medlar decoction. METHODS: The extraction technology and antibacterial activity of the active ingredients of Xiaoxianxiong and Zhishitang were studied by orthogonal test, HPLC analysis technique and antibacterial test. Results: The ratio of water to water 1:4, extraction time 1.5 h, and extraction times were the best extraction conditions; antibacterial MIC: mold> 125 g·L-1, E. coli, Staphylococcus aureus> 62.5 g·L-1. Conclusion: The best condition for extracting the active ingredients of Xiaoxianxiong and Zhishitang by combined water extraction is A1B3C3. All of the extracts have certain antibacterial effect.