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应用基因组原位杂交技术分析了抗小麦黄矮病种质的遗传组成,研究表明小麦-中间偃麦草部分双二倍体无芒中4(2n=56)具有40条小麦染色体、5对中间偃麦草染色体、3对小麦/中间偃麦草易位染色体,其中1对是罗伯逊氏易位染色体。结果表明无芒中4与远中5的遗传组成有明显差异,是两种不同类型的材料。抗黄矮病小麦种质F940418、T103均为抗病易位系,易位片段位于小麦染色体的端部,且为小片段易位。应用RAPD技术筛选出与来自L_1(抗黄矮病异附加系的抗黄矮病基因连锁的分子标记AB-01_(1500),为抗黄矮病育种提供了可靠的选择标记。
The genetic composition of the resistant wheat yellow dwarf germplasm was analyzed by using genomic in situ hybridization. The results showed that 40 diploid wheat chromosomes were found in the diploid diploid and the middle pair Wheatgrass chromosomes, 3 pairs of wheat / Thinopyrum translocation chromosomes, 1 of which is Robertson’s translocation chromosome. The results show that there is a significant difference between the genetic composition of Fusanzhong 4 and Yuanzhong 5, which are two different types of materials. Resistance to yellow dwarf wheat germplasm F940418, T103 are disease-resistant translocations, translocation fragment at the end of wheat chromosomes, and small fragments of translocation. RAPD technique was used to screen the molecular marker AB-01_ (1500) linked to L_1, a resistance gene to yellow dwarf virus, to provide a reliable selection marker for resistance to yellow dwarf disease.