论文部分内容阅读
用于制备单克隆抗体腹水的动物通常为与杂交细胞同源的ＢＡＬＢ／ｃ小鼠。由于该小鼠为近交系，抵抗力差，繁殖困难，故价格较贵。我们参考了Ｂｒｏｄｅｕｒ方法，用本中心生产的４种不同品系雌性小鼠与ＢＡＬＢ／ｃ雄鼠交配生产的杂交一代（Ｆ１）小鼠制备单克隆抗体腹水，并
The animal used to prepare the monoclonal antibody ascites is typically a BALB / c mouse that is homologous to the hybrid cell. As the mouse is inbred line, poor resistance, breeding difficulties, so the price is more expensive. We refer to the Brodeur method to prepare monoclonal antibody ascites using F1 generation mice of 4 different female and BALB / c male mice produced by our institute