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目的观察不同浓度二甲基亚砜(DMSO)对Min6胰岛细胞活力和细胞活性氧族(ROS)产生的影响。方法无菌洁净环境下培养Min6胰岛细胞株,细胞良好生长状态时,分为正常对照组(DMSO含量为0%)及DMSO各浓度组,用不同浓度DMSO(0.005%、0.012 5%、0.05%、0.125%、0.25%)干预Min6胰岛细胞48 h,采用细胞增殖和毒性检测试剂盒(Cell Counting Kit-8,CCK8法)检测细胞活力,采用2’,7’-二氯荧光黄双乙酸盐(DCFH-DA)探针法检测ROS的产生水平。结果与正常对照组比较(不含DMSO),0.005%、0.012 5%、0.05%、0.125%,0.25%DMSO组细胞活力均降低;0.005%、0.012 5%、0.05%、0.125%DMSO组间细胞活力差别不大,0.25%DMSO组细胞活力进一步下降,同时ROS产生明显增加。结论 Min6细胞对DMSO敏感,DMSO浓度水平对细胞活力、ROS水平有一定影响,建议相关实验时注意各组DMSO含量本身齐同可比,以免影响结果判断。
Objective To observe the effects of different concentrations of dimethyl sulfoxide (DMSO) on Min6 islet cell viability and reactive oxygen species (ROS) production. Methods Min6 islet cells were cultured under aseptic and clean conditions. When the cells grew well, the cells were divided into normal control group (DMSO content 0%) and DMSO concentration groups. DMSO (0.005%, 0.012 5%, 0.05% , 0.125%, 0.25%) for 48 h, cell viability was detected by Cell Counting Kit-8 (CCK8), and cell viability was measured by using 2 ’, 7’-dichlorofluorescein diacetate DCFH-DA) probe to detect the level of ROS production. Results Compared with normal control group (DMSO-free), the viability of cells in 0.005%, 0.012 5%, 0.05%, 0.125% and 0.25% DMSO groups were decreased. Cells in 0.005%, 0.012 5%, 0.05% and 0.125% There was no significant difference in viability between the two groups. The viability of cells in 0.25% DMSO group decreased further and ROS production increased significantly. Conclusion Min6 cells are sensitive to DMSO. The concentration of DMSO has certain influence on the cell viability and ROS level. It is suggested that the DMSO content in each group should be comparable with each other in order to avoid affecting the result judgment.