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目的:了解西双版纳州肠出血性大肠杆菌O157:H7动物宿主带菌情况,为制订防制策略提供依据。方法:采用免疫磁珠富集法,对282份入户采集的猪、牛、鸡、鸭、鹅、羊、鸽新鲜粪便标本进行肠出血性大肠杆菌O157:H7检测。结果:共检出肠出血性大肠杆菌O157:H7阳性标本27份,动物宿主的带菌率为9.57%。其中,牛带菌率最高为15.05%,鸭带菌率为13.64%,猪带菌率为9.41%,鸡带菌率为3.45%,鹅、羊、鸽动物宿主未检出肠出血性大肠杆菌O157:H7。结论:西双版纳州动物宿主中存在肠出血性大肠杆菌O157∶H7感染,具有潜在的流行性威胁。
Objective: To understand the host bacteriostasis of enterohemorrhagic Escherichia coli O157: H7 in Xishuangbanna Prefecture and provide the evidence for the development of control strategy. Methods: Immunomagnetic beads enrichment method was used to detect enterohemorrhagic Escherichia coli O157: H7 in 282 samples of fresh stool samples from pigs, cattle, chickens, ducks, geese, sheep and pigeons collected at home. Results: Twenty-seven positive samples of enterohemorrhagic Escherichia coli O157: H7 were detected, and the carriage rate of animal hosts was 9.57%. Among them, the highest rate of bovine band was 15.05%, the rate of duck band was 13.64%, the rate of pig banding was 9.41% and the rate of banding was 3.45%. The enterohemorrhagic Escherichia coli O157: H7 was not detected in the goose, sheep and pigeon animal hosts. Conclusion: Enterohemorrhagic Escherichia coli O157: H7 infection exists in animal hosts in Xishuangbanna Prefecture, which has potential epidemic threat.