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本文报告用胃液GCAA粗提液免疫家兔,制备抗血清,经亲和层析法纯化后,检测了160例患者,测得胃癌的阳性检出率为88.6％,假阳性率3.4％。本试验证实:胃液GCAA是一种对胃癌有一定特异性和敏感性的肿瘤标记,可用于胃癌的辅助性诊断。提纯后的胃液GCAA,琼脂免疫电泳位于β区,在聚丙烯酰胺凝胶圆盘电泳上显示出一条带。胃液GCAA仅存在于胃液中,不分泌进入血液。胃液GCAA的检出不受肿瘤部位和组织学类型的影响。伴有肠上皮化生的病例,胃液GCAA的检出率显著高于不伴有肠上皮化生的病例,胃液GCAA不仅可以检出胃癌,也可以检出癌前期病变。
In this report, rabbits were immunized with gastric juice GCAA crude extracts to prepare antiserum. After purification by affinity chromatography, 160 patients were detected. The positive detection rate of gastric cancer was 88.6%, and the false positive rate was 3.4%. This test confirms that GCAA is a tumor marker with specificity and sensitivity to gastric cancer and can be used for the adjuvant diagnosis of gastric cancer. Purified gastric juice GCAA, agar electrophoresis located in the beta region, showed a band on the polyacrylamide gel disc electrophoresis. Gastric fluid GCAA is only present in gastric juice and is not secreted into the blood. Gastric fluid GCAA detection was not affected by tumor site and histological type. In patients with intestinal metaplasia, the GCAA detection rate in gastric juice was significantly higher than that in patients without intestinal metaplasia. Gastric juice GCAA can not only detect gastric cancer, but also detect precancerous lesions.