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目的建立柴胡疏肝汤高效液相指纹图谱方法,并同时测定柴胡皂苷a、汉黄芩苷、芍药苷的含量,为该方质量控制提供参考。方法采用RP-HPLP-DAD法,利用ZORBAX SB-C8色谱柱(250 mm×4.6 mm,5μm),在检测波长210nm、柱温25℃下,以乙腈(A)-0.2%磷酸水溶液(B)为流动相进行梯度洗脱。对10批柴胡疏肝汤进行指纹图谱及柴胡皂苷a、汉黄芩苷、芍药苷含量测定研究,采用“中药色谱指纹谱图相似度评估软件”(2004A)进行分析。结果建立了柴胡疏肝汤指纹图谱共有模式,标定22个共有峰,10批柴胡疏肝汤相似度较好(均大于0.96);通过与标准品的保留时间和紫外光谱图对比,确定了7个成分;柴胡皂苷a、汉黄芩苷、芍药苷重复性、稳定性良好,含量测定结果可靠。结论指纹图谱的建立和多成分含量测定,可以为该方提供较全面的质量控制依据。
Objective To establish a method of HPLC fingerprint of Chaihu Shugan Decoction and to determine the contents of saikosaponin a, baicalin and paeoniflorin simultaneously, which may provide a reference for the quality control of this prescription. Methods RP-HPLP-DAD method was used to determine the content of nitrite in aqueous solution of acetonitrile (A) -0.2% phosphoric acid solution (B) at a detection wavelength of 210 nm and a column temperature of 25 ° C using a ZORBAX SB-C8 column (250 mm × 4.6 mm, Gradient elution of the mobile phase. Fingerprints of 10 batches of Chaihu Shugan Decoction and determination of saikosaponin a, baicalin and paeoniflorin were carried out and analyzed using the software of similarity assessment of chromatographic fingerprints of traditional Chinese medicines (2004A). Results The common pattern of fingerprint of Chaihu Shugan Tang was established and 22 common peaks were calibrated. The similarity of 10 batches of Chaihu Shugan Decoction was better (all greater than 0.96). By comparing with the standard retention time and ultraviolet spectrum, 7 components; Saikosaponin a, baicalin, paeoniflorin repeatability, good stability, the determination of the content of the results. Conclusion The establishment of fingerprint and determination of multi-component content can provide a more comprehensive quality control basis for this side.